[Histonet] Hi, We have recently started staining liver biopsies routinely with Retic (untoned, no counterstain). We are trying to achieve black reticulin fibres with a clear background. Although we produce clean, crisp Retics we are still left with a golden background. We use Sodium Thiosulphate, does anyone have any other ideas on solutions that would remove this background stain?

[SARAH REEVES]

Hi, We have recently started staining liver biopsies routinely with Retic (untoned, no counterstain).  We are trying to achieve black reticulin fibres with a clear background.  Although we produce clean, crisp Retics we are still left with a golden background. We use Sodium Thiosulphate, does anyone have any other ideas on solutions that would remove this background stain?

Thanks in advance

Sarah Reeves


**********************************************************************
This email and any files transmitted with it are confidential and intended
solely for the use of the individual or entity to whom they are addressed.
Any views or opinions presented are solely those of the author and do 
not necessarily represent those of East Kent Hospitals University NHS Trust.  If
you are not the intended recipient, be advised that you have received
this email in error and that any use, dissemination, forwarding, printing
or copying of this email is strictly prohibited.

If you have received this email in error please notify the system
manager at the following email address: root.postmaster <@t> ekht.nhs.uk

www.kentandmedway.nhs.uk

This footnote also confirms that although this email message has been
swept by MIMEsweeper for the presence of computer viruses, it is strongly 
recommended that you carry out your own virus scan of this
message and any attachments.

www.mimesweeper.com
**********************************************************************