[Histonet] CD68 counts

[Mark Tarango]

Well, you're going to have dendritic cells staining with CD68.  Dendritic
cells be really large, full of cytoplasm, and multinucleated.

I guess that brings you back to "hopefully macrophages".  Maybe someone can
recommend a more specific marker or better criteria for what a macrophage
is...

Mark


On Wed, Jan 21, 2009 at 8:48 AM, Edwards, R.E. <ree3 <@t> leicester.ac.uk> wrote:

>
> I am attempting to accurately assess the number of CD 68 positive cells
> (hopefully macrophages) in biopsies,  they have been GMA processed, ABC
> staining, with AEC visualisation;  no problem with a nucleated cell with
> positive cell  membrane, but  they  are  in  the minority, so  the  question
> how  does one judge the cut-off point that makes a positive cell  into a
> negative cell  if you catch my drift??. I am quite experienced at counting
> cells but feel that obtaining reliable, consistent results from this
> material is quite challenging.
>               All ideas/thoughts gratefully received
>
>                         Cheers
>                                Richard Edwards
>                                  University of Leicester
>                                            U.K.
>
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