[Histonet] staining rat tissue with monoclonal antibody?

[Andrea Hooper]

Just make sure you use an anti-mouse IgG antibody that is not cross 
reactive to rat IgG. Jackson ImmunoResearch is the best place to find 
these reagents ... I suggest the donkey as host, it works best for 
me, least background. Beautiful results ...

>Good afternoon.
>I just had someone ask me about staining rat tissue using an 
>monoclonal (mouse) antibody. I have never done this before and so 
>was wondering if there is anything in particular I need to keep in 
>mind. Does mouse antibodies cause a problem when staining rat 
>tissues? Do I have to use a specific kit or specific secondary 
>antibody? Can I use a biotinylated goat-anti-mouse secondary 
>followed by ABC?
>Thanks for your help,
>Eva

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