[Histonet] staining rat tissue with monoclonal antibody?

[Pritchard, Michele]

Hi:

We routinely perform immunohistochemistry (IHC) to detect antigens on
rat tissues.  A mouse mAb should be fine for use in your rat samples.
You will not need a special kit, just an anti-mouse secondary Ab.  One
runs into trouble when they use a mouse (or rat) primary on mouse (or
rat) tissue, respectively.  Then there are kits (i.e. Mouse-on-Mouse
kits) that can decrease background staining due to the anti-mouse IgG
secondary you would need to use to detect the primary Ab.  From my
limited knowledge of IHC, I would say that you could certainly use a
biotinylated goat-anti-mouse secondary followed by ABC.  I do this all
the time and use DAB for visualization of my antigen of interest.

Cheers:

---mtp

Michele T. Pritchard, Ph.D.
Research Associate
Nagy Laboratory
Department of Pathobiology/NE40
Lerner Research Institute
Cleveland Clinic
9500 Euclid Avenue
Cleveland, OH 44195
 
phone:  216.444.8613
fax:  216.636.1493
 
email:  pritchm <@t> ccf.org
 
Lab location:
Lerner Research Institute
NE4-214

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Eva
Permaul
Sent: Wednesday, January 14, 2009 2:56 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] staining rat tissue with monoclonal antibody?

Good afternoon.
I just had someone ask me about staining rat tissue using an monoclonal 
(mouse) antibody. I have never done this before and so was wondering if 
there is anything in particular I need to keep in mind. Does mouse 
antibodies cause a problem when staining rat tissues? Do I have to use a

specific kit or specific secondary antibody? Can I use a biotinylated 
goat-anti-mouse secondary followed by ABC?
Thanks for your help,
Eva

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