[Histonet] staining rat tissue with monoclonal antibody?

[Bernice Frederick]

We try and find a mouse anti-rat antibody if we can and run rat secondary
(Dako makes one)
Bernice


Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL 
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Eva Permaul
Sent: Wednesday, January 14, 2009 1:56 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] staining rat tissue with monoclonal antibody?

Good afternoon.
I just had someone ask me about staining rat tissue using an monoclonal 
(mouse) antibody. I have never done this before and so was wondering if 
there is anything in particular I need to keep in mind. Does mouse 
antibodies cause a problem when staining rat tissues? Do I have to use a 
specific kit or specific secondary antibody? Can I use a biotinylated 
goat-anti-mouse secondary followed by ABC?
Thanks for your help,
Eva

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