[Histonet] Spit and Diastase - No more expectations of expectorations

[Tony Henwood]

My staff refused to use spit to do a DiPAS so we developed an
alternative (Lasts months at 4oC - just pipette or using a dropper, add
to the slides & incubate 10min at room temp). See:

Mangan, V-M, Farago, V., Kelly, M., Henwood, A.F., (2002) "An Amylase
Reagent with a Long Shelf Life for the removal of Glycogen from Tissue
Sections" J. Histotechnol 25:153.

No more expectations of expectorations (ie no more spitting) in the lab.

Possibly the staff member expectorated on the wrong side of the slide.



Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Parker,
Helayne
Sent: Wednesday, 14 January 2009 4:37 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Spit and Diastase


Hi Gang,
   What would cause someone's spit to no longer work while doing a PAS
w/ Diatase. She is a trainee and has done this several times prev to
this w/o any issues and all the sudden her spit does not work.  Did not
work yesterday nor did the repeat today work.  Well off to repeat it
again w/ my spit.  

Any suggestions,
Thanks
Helayne Parker

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