[Histonet] Spit and Diastase

[Rene J Buesa]

Spitting to do a PAS "with diastase" is repugnant and unhealthy. Also it may contaminate the sections with mouth bacteria.
Use a pig diastase, that is not as "readily available as the saliva you carry around with you" but for certain more becoming.
Are you going to write in your SOP that s/he who is doing this procedure is going to spit over the section? How much saliva? Before or after eating?
It is just revolting and disgusting!
René J.

--- On Tue, 1/13/09, Parker, Helayne <HParker <@t> Skaggs.Net> wrote:

From: Parker, Helayne <HParker <@t> Skaggs.Net>
Subject: [Histonet] Spit and Diastase
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, January 13, 2009, 12:37 PM

Hi Gang,
   What would cause someone's spit to no longer work while doing a PAS
w/ Diatase. She is a trainee and has done this several times prev to
this w/o any issues and all the sudden her spit does not work.  Did not
work yesterday nor did the repeat today work.  Well off to repeat it
again w/ my spit.  

Any suggestions,
Thanks
Helayne Parker

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