[Histonet] Nuclear fast red
Hobbs, Carl
carl.hobbs <@t> kcl.ac.uk
Thu Feb 26 13:56:23 CST 2009
I agree with Geoff re NFRed staining solution.
I have always had good results with home-made NFRed, if only for 2/4 months.
Much cheaper too.
Sure, try Neutral red: differentiate in water until you like the contrast then, blot dry your section and immerse in Xylene after a time that you think has made that section dry....then mount in DPX, for eg
You do not give any details re your Preps: So.....
Could be cells/sections
Could be fixation problems
Could be technical errors.
Could be old solution of NFR
?
carlos
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu [histonet-request <@t> lists.utsouthwestern.edu]
Sent: 26 February 2009 18:02
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 63, Issue 49
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Today's Topics:
1. Tube station (Marcia Funk)
2. RE: Tube station (Mike Pence)
3. RE: Tube station (Horn, Hazel V)
4. Plant Histology (pam plumlee)
5. Re: Mohs technique (whitmorel)
6. AW: [Histonet] Tube station (Gudrun Lang)
7. RE: Tube station (Della Speranza, Vinnie)
8. RE: Tube station (Bernice Frederick)
9. Re: Tube station (Emily Sours)
10. RE: Tube station (Bonner, Janet)
11. Re: Tube station (Peter Carroll)
12. Nuclear fast red (Sharon.Davis-Devine)
13. RE: Nuclear fast red (Jodie Robertson)
14. RE: Nuclear fast red (Amspacher, September)
15. Re: Nuclear fast red (Geoff McAuliffe)
16. muscle skinning solution for fluorescence microscopy
(Matthew T Close)
17. Re: Nuclear fast red (nancy lowen)
18. RE: Nuclear fast red (Smith, Allen)
19. slide storage pages (louise renton)
20. RE: Tube station (Edwards, R.E.)
21. Vegetal Histology. (Sebasti?n Vecchio)
22. Triton x-100 stability (Beno?t Delatour)
23. RE: slide storage pages (Swain, Frances L)
24. PAI-1 antibody needed (Alexandra Meinl)
25. Local PRN and Contract in Northern NJ/Greater NYC area!
(Kyla Nemitz)
26. RELIA Histology Careers Bulletin 2/26/09 Are you in your
dream job? (Pam Barker)
----------------------------------------------------------------------
Message: 1
Date: Wed, 25 Feb 2009 13:32:32 -0500
From: "Marcia Funk" <FUNKM <@t> mercyhealth.com>
Subject: [Histonet] Tube station
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <49A53A60.9B87.00AC.0 <@t> mercyhealth.com>
Content-Type: text/plain; charset=US-ASCII
We have just installed a tube station do you send your tissue specimens through the tube
station ? Thanks Marcia
Marcia Funk
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
------------------------------
Message: 2
Date: Wed, 25 Feb 2009 12:53:00 -0600
From: "Mike Pence" <mpence <@t> grhs.net>
Subject: RE: [Histonet] Tube station
To: "Marcia Funk" <FUNKM <@t> mercyhealth.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<661949901A768E4F9CC16D8AF8F2838C017A3AB8 <@t> IS-E2K3.grhs.net>
Content-Type: text/plain; charset="us-ascii"
We have a policy that state what items can and cannot be tubed and
tissue specimens are one that we do not allow to be tubed.
Mike
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marcia
Funk
Sent: Wednesday, February 25, 2009 12:33 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Tube station
We have just installed a tube station do you send your tissue
specimens through the tube
station ? Thanks Marcia
Marcia Funk
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 3
Date: Wed, 25 Feb 2009 12:55:33 -0600
From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
Subject: RE: [Histonet] Tube station
To: "Marcia Funk" <FUNKM <@t> mercyhealth.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9AE8AA9E1F644B4AA6C155FB6FD51C6317D83073 <@t> EMAIL.archildrens.org>
Content-Type: text/plain; charset="us-ascii"
We do not allow formalin fixed specimens to be tubed.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's Way Slot 820
Little Rock, AR 72202
phone 501.364.4240
fax 501.364.3155
visit us on the web at: www.archildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marcia
Funk
Sent: Wednesday, February 25, 2009 12:33 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Tube station
We have just installed a tube station do you send your tissue
specimens through the tube
station ? Thanks Marcia
Marcia Funk
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
******************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************
The information contained in this message may be privileged and confidential
and protected from disclosure. If the reader of this message is not the
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Thank you.
------------------------------
Message: 4
Date: Wed, 25 Feb 2009 11:11:22 -0800 (PST)
From: pam plumlee <paw555 <@t> yahoo.com>
Subject: [Histonet] Plant Histology
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <479984.42273.qm <@t> web31906.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi Histonetters: I was hoping someone could share a
simple plant tissue (green stem and buds) processing
program with me. I have never done plant histology,
but would like to earn some extra credit for a
horticulture class I'm taking. Thanks,
Pam Plumlee H.T.
------------------------------
Message: 5
Date: Wed, 25 Feb 2009 14:21:15 -0500 (EST)
From: whitmorel <whitmorel <@t> mindspring.com>
Subject: [Histonet] Re: Mohs technique
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<23409049.1235589675823.JavaMail.root <@t> elwamui-lapwing.atl.sa.earthlink.net>
Content-Type: text/plain; charset=UTF-8
First of all, there is no apostrophe in Mohs. Sorry, being picky is a necessity for a Mohs tech. How are you mounting your tissue? Are you using a glass slide to mount the tissue? There are several different method out there for mounting tissue so you can be sure the entire epidermis is completely down and air bubbles are out of the tissue. You might have your surgeon contact the Mohs College and see about getting a trainer to come and work with you. With your background, the 2 days the College suggests would work great. The other alternative is to go and visit a trainer. If you go onto the website www.mohscollege.org you can find a list of Mohs histotech trainers.
Lynn Whitmore HT(ASCP)
Mohs Histotechnology Trainer
-
>------------------------------
>
>Message: 17
>Date: Wed, 25 Feb 2009 06:56:12 -0800 (PST)
>From: Steven Coakley <sjchtascp <@t> yahoo.com>
>Subject: [Histonet] Moh's techniques
>To: Histonet <@t> lists.utsouthwestern.edu
>Message-ID: <164204.1533.qm <@t> web38206.mail.mud.yahoo.com>
>Content-Type: text/plain; charset=iso-8859-1
>
>Good morning all,
>??
>I'm learning a new way to do Moh's.?? Much more relaxed compared to how I did them years ago with a Pathologist looking over my shoulder while I attempted to cryosection 12, 3,9,12 o'clock boarders, stain them by hand and the Dermatologist wanting the results "yesterday".?? I'd like????to get some ideas as to techniques Moh's Techs are using out there that work well in assuring that one gets the entire skin edge.?? I'd also Like to shadow in any fairly local Moh's labs in the So.WI or No. Ill. area.
>??
>Thanks everyone,
>??
>Steve
>??
>
>
>
>
>------------------------------
>
>M
------------------------------
Message: 6
Date: Wed, 25 Feb 2009 20:24:51 +0100
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] Tube station
To: "'Marcia Funk'" <FUNKM <@t> mercyhealth.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <6AC9DD87387B4B33AD121A4E7EE50126 <@t> dielangs.at>
Content-Type: text/plain; charset="iso-8859-1"
We let unfixed specimen for frozens send through the tube. And also specimen
in formalin with tight caps and small enough for the tube. The container are
packed in extra bags and with blister-plastic.
Gudrun
-----Urspr?ngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Marcia
Funk
Gesendet: Mittwoch, 25. Februar 2009 19:33
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Tube station
We have just installed a tube station do you send your tissue
specimens through the tube
station ? Thanks Marcia
Marcia Funk
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Wed, 25 Feb 2009 14:26:42 -0500
From: "Della Speranza, Vinnie" <dellav <@t> musc.edu>
Subject: RE: [Histonet] Tube station
To: "'Marcia Funk'" <FUNKM <@t> mercyhealth.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E58D1CD977E29C46A167806513B04577862A7610ED <@t> EVS5.clinlan.local>
Content-Type: text/plain; charset="us-ascii"
We do not tube any tissue samples, fresh or fixed, and yes, we do have a tube station available to us.
Potential contamination of the tube system from a leaking specimen container is one legitimate concern but we also have some here worried that a fresh specimen might enter the tube system never to be seen again. I'm not sure how realistic that fear is with modern systems that track each transaction however I'm reluctant to find out the hard way. Emotions aside, as a matter of policy we do not tube any tissues.
Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marcia Funk
Sent: Wednesday, February 25, 2009 1:33 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Tube station
We have just installed a tube station do you send your tissue specimens through the tube
station ? Thanks Marcia
Marcia Funk
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Wed, 25 Feb 2009 14:07:03 -0600
From: "Bernice Frederick" <b-frederick <@t> northwestern.edu>
Subject: RE: [Histonet] Tube station
To: "'Della Speranza, Vinnie'" <dellav <@t> musc.edu>, "'Marcia Funk'"
<FUNKM <@t> mercyhealth.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <CDD574F3B7BD40BD833CE1BADB3C04CB <@t> lurie.northwestern.edu>
Content-Type: text/plain; charset="us-ascii"
Ever since a leaky container and a biohazard bag opened in the tube
containing bile- No Way. They had to shut the system down to clean it. And
it went across the street and up 3 floors! University of Chicago has a
dumbwaiter that sends samples from surgery to the gross room. Nifty!!!
Bernice
Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Della
Speranza, Vinnie
Sent: Wednesday, February 25, 2009 1:27 PM
To: 'Marcia Funk'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Tube station
We do not tube any tissue samples, fresh or fixed, and yes, we do have a
tube station available to us.
Potential contamination of the tube system from a leaking specimen container
is one legitimate concern but we also have some here worried that a fresh
specimen might enter the tube system never to be seen again. I'm not sure
how realistic that fear is with modern systems that track each transaction
however I'm reluctant to find out the hard way. Emotions aside, as a matter
of policy we do not tube any tissues.
Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marcia Funk
Sent: Wednesday, February 25, 2009 1:33 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Tube station
We have just installed a tube station do you send your tissue
specimens through the tube
station ? Thanks Marcia
Marcia Funk
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Wed, 25 Feb 2009 15:17:35 -0500
From: Emily Sours <talulahgosh <@t> gmail.com>
Subject: Re: [Histonet] Tube station
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<b39794b0902251217v57c0fec3kcb1fe65e8f9f1009 <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
A tube station?!
Is that like the internet--a series of connected tubes?!
Emily
On Wed, Feb 25, 2009 at 1:32 PM, Marcia Funk <FUNKM <@t> mercyhealth.com> wrote:
>
> We have just installed a tube station do you send your tissue
> specimens through the tube
> station ? Thanks Marcia
>
> Marcia Funk
> Histology Laboratory
> Mercy Medical Center North Iowa
> Mason City, IA, 50401
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
It's like hearing Billy Joel play "Piano Man"-- joyless for all involved,
but demanded by a higher power.
--Kevin Murphy, Indiana Jones and the Crystal Skull rifftrax
------------------------------
Message: 10
Date: Wed, 25 Feb 2009 15:27:22 -0500
From: "Bonner, Janet" <Janet.Bonner <@t> FLHOSP.ORG>
Subject: RE: [Histonet] Tube station
To: "Marcia Funk" <FUNKM <@t> mercyhealth.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<5F31F38C96781A4FBE3196EBC22D47807F2921 <@t> fhosxchmb006.ADVENTISTCORP.NET>
Content-Type: text/plain; charset=iso-8859-1
No, the patient tissue is too precious to "loose" should the tube get stuck, and the legal implications are well.....
Nor do you want a hazardous fluid such as Formalin to spill within the tube system.
So we just don't. We have couriers to go among the departments or our staff will do the pick-up.
The tube system is great for paper items, addressograph labels, or if a floor/department needs a form.
Janet
Janet L. Bonner, HTL (ASCP)
Pathology Laboratory
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Marcia Funk
Sent: Wed 2/25/2009 1:32 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Tube station
We have just installed a tube station do you send your tissue specimens through the tube
station ? Thanks Marcia
Marcia Funk
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
=======================================================
The information contained in this message may be privileged and/or confidential
and protected from disclosure. If the reader of this message is not the intended
recipient or an employee or agent responsible for delivering this message to the
intended recipient, you are hereby notified that any dissemination, distribution
or copying of this communication is strictly prohibited. If you have received this
communication in error, please notify the sender immediately by replying to this
message and deleting the material from any computer.
=======================================================
------------------------------
Message: 11
Date: Wed, 25 Feb 2009 15:28:59 -0500
From: Peter Carroll <carrolpb <@t> umdnj.edu>
Subject: Re: [Histonet] Tube station
To: Emily Sours <talulahgosh <@t> gmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <49A5AA0B.80906 <@t> umdnj.edu>
Content-Type: text/plain; charset=UTF-8; format=flowed
> Is that like the internet--a series of connected tubes?!
Nope, its more like this.... http://en.wikipedia.org/wiki/Tube_station
------------------------------
Message: 12
Date: Wed, 25 Feb 2009 15:11:14 -0600
From: "Sharon.Davis-Devine" <Sharon.Davis-Devine <@t> carle.com>
Subject: [Histonet] Nuclear fast red
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<44780C571F28624DBB446DE55C4D733A04257C61 <@t> EXCHANGEBE1.carle.com>
Content-Type: text/plain; charset="us-ascii"
We have ordered Nuclear Fast Red from both EMS and Sigma and neither one
of them seem to work very well. Both appear to have very weak staining.
Is there someone out there in Histoland that could lead us to Nuclear
Fast Red that actually works? Thanks is advance for all of your
recommendations.
Sharon Davis-Devine, CT (ASCP)
Cytology Supervisor
Carle Clinic
602 West University
Urbana, Illinois 61801
Phone: 217-383-3572
Email: sharon.davis-devine <@t> carle.com
------------------------------
Message: 13
Date: Wed, 25 Feb 2009 13:16:05 -0800
From: "Jodie Robertson" <jrobertson <@t> pathologysciences.com>
Subject: RE: [Histonet] Nuclear fast red
To: "Sharon.Davis-Devine" <Sharon.Davis-Devine <@t> carle.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<518CD6920AA7154193CBE5977CD88073177EC1 <@t> psmgsrv2.PSMG.local>
Content-Type: text/plain; charset="us-ascii"
Try Newcomer Supply.
Jodie Robertson, HT (ASCP) QIHC
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Sharon.Davis-Devine
Sent: Wednesday, February 25, 2009 1:11 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Nuclear fast red
We have ordered Nuclear Fast Red from both EMS and Sigma and neither one
of them seem to work very well. Both appear to have very weak staining.
Is there someone out there in Histoland that could lead us to Nuclear
Fast Red that actually works? Thanks is advance for all of your
recommendations.
Sharon Davis-Devine, CT (ASCP)
Cytology Supervisor
Carle Clinic
602 West University
Urbana, Illinois 61801
Phone: 217-383-3572
Email: sharon.davis-devine <@t> carle.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Wed, 25 Feb 2009 16:18:07 -0500
From: "Amspacher, September" <september.amspacher <@t> bassett.org>
Subject: RE: [Histonet] Nuclear fast red
To: "Sharon.Davis-Devine" <Sharon.Davis-Devine <@t> carle.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <9485D6C8CD957E488B81B56B0EBB28A44F454B <@t> ex3.bassett.org>
Content-Type: text/plain; charset="us-ascii"
Newcomer or American Master Tech
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Sharon.Davis-Devine
Sent: Wednesday, February 25, 2009 4:11 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Nuclear fast red
We have ordered Nuclear Fast Red from both EMS and Sigma and neither one
of them seem to work very well. Both appear to have very weak staining.
Is there someone out there in Histoland that could lead us to Nuclear
Fast Red that actually works? Thanks is advance for all of your
recommendations.
Sharon Davis-Devine, CT (ASCP)
Cytology Supervisor
Carle Clinic
602 West University
Urbana, Illinois 61801
Phone: 217-383-3572
Email: sharon.davis-devine <@t> carle.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Wed, 25 Feb 2009 16:22:34 -0500
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] Nuclear fast red
To: "Sharon.Davis-Devine" <Sharon.Davis-Devine <@t> carle.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <49A5B69A.9080309 <@t> umdnj.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Hi Sharon:
This is a common complaint with this stain. Some (not me) have had good
results with "Brizillant" from Anatech.
Geoff
Sharon.Davis-Devine wrote:
> We have ordered Nuclear Fast Red from both EMS and Sigma and neither one
> of them seem to work very well. Both appear to have very weak staining.
> Is there someone out there in Histoland that could lead us to Nuclear
> Fast Red that actually works? Thanks is advance for all of your
> recommendations.
>
>
>
>
>
> Sharon Davis-Devine, CT (ASCP)
>
> Cytology Supervisor
>
> Carle Clinic
>
> 602 West University
>
> Urbana, Illinois 61801
>
> Phone: 217-383-3572
>
> Email: sharon.davis-devine <@t> carle.com
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff <@t> umdnj.edu
**********************************************
------------------------------
Message: 16
Date: Wed, 25 Feb 2009 16:47:16 -0500 (EST)
From: Matthew T Close <mtc205 <@t> Lehigh.EDU>
Subject: [Histonet] muscle skinning solution for fluorescence
microscopy
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20819464.1235598436924.JavaMail.mtc205 <@t> lehigh.edu>
Content-Type: text/plain; charset="UTF-8"
I am in need of a good, working chemical skinnig technique for
skeletal muscle fibers. I will be staining skinned, isolated fibrils
with Rh-Phalloidin and DAPI stains for observation and analysis using
a fluorescence scope. Thanks.
---------------------------------
Matthew T. Close Lehigh University Department of Biological Sciences
------------------------------
Message: 17
Date: Wed, 25 Feb 2009 13:48:20 -0800 (PST)
From: nancy lowen <claycal44 <@t> yahoo.com>
Subject: Re: [Histonet] Nuclear fast red
To: histonet <@t> lists.utsouthwestern.edu, "Sharon.Davis-Devine"
<Sharon.Davis-Devine <@t> carle.com>
Message-ID: <168016.97490.qm <@t> web65613.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Accurate Chemical and Scientific Corp.? 50 ml. bottles.? Our VWR rep found it for us.
Nancy
Nancy.lowen <@t> med.va.gov
--- On Wed, 2/25/09, Sharon.Davis-Devine <Sharon.Davis-Devine <@t> carle.com> wrote:
From: Sharon.Davis-Devine <Sharon.Davis-Devine <@t> carle.com>
Subject: [Histonet] Nuclear fast red
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, February 25, 2009, 1:11 PM
We have ordered Nuclear Fast Red from both EMS and Sigma and neither one
of them seem to work very well. Both appear to have very weak staining.
Is there someone out there in Histoland that could lead us to Nuclear
Fast Red that actually works? Thanks is advance for all of your
recommendations.
Sharon Davis-Devine, CT (ASCP)
Cytology Supervisor
Carle Clinic
602 West University
Urbana, Illinois 61801
Phone: 217-383-3572
Email: sharon.davis-devine <@t> carle.com
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 18
Date: Wed, 25 Feb 2009 19:26:04 -0500
From: "Smith, Allen" <asmith <@t> mail.barry.edu>
Subject: [Histonet] RE: Nuclear fast red
To: 'Sharon.Davis-Devine' <Sharon.Davis-Devine <@t> carle.com>
Cc: "'Histonet <@t> lists.utsouthwestern.edu'"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E4132130AC2F764D8C173C5400D53042A202EB035D <@t> exchsrv02.barrynet.barry.edu>
Content-Type: text/plain; charset="us-ascii"
I bought my nuclear fast red from Aldrich 15 years ago. Kernechtrot (nuclear fast red with an aluminum mordant) solutions made from it still work well and still last about a year.
Allen A. Smith, Ph.D.
Barry University School of Podiatric Medicine
Miami Shores, Florida
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine
Sent: Wednesday, February 25, 2009 4:11 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Nuclear fast red
We have ordered Nuclear Fast Red from both EMS and Sigma and neither one
of them seem to work very well. Both appear to have very weak staining.
Is there someone out there in Histoland that could lead us to Nuclear
Fast Red that actually works? Thanks is advance for all of your
recommendations.
Sharon Davis-Devine, CT (ASCP)
Cytology Supervisor
Carle Clinic
602 West University
Urbana, Illinois 61801
Phone: 217-383-3572
Email: sharon.davis-devine <@t> carle.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 19
Date: Thu, 26 Feb 2009 11:27:04 +0200
From: louise renton <louise.renton <@t> gmail.com>
Subject: [Histonet] slide storage pages
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<e483362e0902260127g819cab8o6ec08fdad8901bb5 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
hi guys perhaps I wasn't that clear in my query. the sheets I am looking for
are for storing microscopy slides, not photo ones....sorry bout that. hope
there are still some suggestions out there
--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
------------------------------
Message: 20
Date: Thu, 26 Feb 2009 09:32:40 +0000
From: "Edwards, R.E." <ree3 <@t> leicester.ac.uk>
Subject: RE: [Histonet] Tube station
To: 'Emily Sours' <talulahgosh <@t> gmail.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<7722595275A4DD4FA225B92CDBF174A17455BB4DE6 <@t> EXC-MBX3.cfs.le.ac.uk>
Content-Type: text/plain; charset="utf-8"
There is only one tube station, Mornington Crescent!!.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: 25 February 2009 20:18
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Tube station
A tube station?!
Is that like the internet--a series of connected tubes?!
Emily
On Wed, Feb 25, 2009 at 1:32 PM, Marcia Funk <FUNKM <@t> mercyhealth.com> wrote:
>
> We have just installed a tube station do you send your tissue
> specimens through the tube
> station ? Thanks Marcia
>
> Marcia Funk
> Histology Laboratory
> Mercy Medical Center North Iowa
> Mason City, IA, 50401
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
It's like hearing Billy Joel play "Piano Man"-- joyless for all involved,
but demanded by a higher power.
--Kevin Murphy, Indiana Jones and the Crystal Skull rifftrax
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------------------------------
Message: 21
Date: Thu, 26 Feb 2009 10:26:42 -0200
From: Sebasti?n Vecchio <boreades <@t> gmail.com>
Subject: [Histonet] Vegetal Histology.
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<8546b4020902260426o6cfd2ad3i66e138502d37ef6b <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi histonetters!! Can any one recommendme a few good resources in vegetal
histology, mainly in techniques to processing vegetals.
Thanks in advance
Sebastian.
------------------------------
Message: 22
Date: Thu, 26 Feb 2009 14:06:47 +0100
From: Beno?t Delatour <benoit.delatour <@t> u-psud.fr>
Subject: [Histonet] Triton x-100 stability
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <49A693E7.4090503 <@t> u-psud.fr>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Dear Histoneters,
We have recently experienced some troubles in carrying out routine
immuno against nuclear fos protein (unexpected weak signal). We have
troubleshooted all what was possible to troubleshoot (use of 3 different
primary antibodies batches, two secondary ab, new buffers, change the
revelation kit etc.). Immunos directed against other epitopes (eg
amyloid-beta) give satisfactory results but the IHC in trouble, that
targets intranuclear epitopes, is still defective. I suspect now that
the non-ionic surfactant we are using (Triton x-100) could have been
degraded by high temperatures we had for several weeks in the lab (due
to a central heating problem in our building). I made some temp.
measurements in the reactives cupboard (around 35-40?C). Does anyone
have any information on Triton x-100 stability? According to
manufacturers' recommendations, the product should be stored at RT but I
did not succeed to get more precise information.
I thank you in advance for all comments, suggestions and alternatives.
B. Delatour
--
Laboratoire de Neurobiologie de l'Apprentissage,
de la M?moire & de la Communication,
NAMC, CNRS UMR 8620, B?t 446
Universit? Paris-Sud
91405 Orsay Cedex, FRANCE
Email benoit.delatour <@t> u-psud.fr
Web http://www.namc.u-psud.fr
If you think research is expensive, try disease.
------------------------------
Message: 23
Date: Thu, 26 Feb 2009 07:23:05 -0600
From: "Swain, Frances L" <SwainFrancesL <@t> uams.edu>
Subject: RE: [Histonet] slide storage pages
To: "louise renton" <louise.renton <@t> gmail.com>,
"Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5B6165D78AC14544974A844787B47E3802AE8D0BAF <@t> MAIL5.ad.uams.edu>
Content-Type: text/plain; charset=us-ascii
Hi Louise: There are two companies that I know of that sell those Microscope Slide viewing and storage sheets. They are Ted Pella Catalog #2111-10 and ProSciTech catalog # H172 they are in Australia. Ted Pella also has the binder to keep them in. I think that ProSciTech does also.
Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic Surgery
Center for Orthopaedic Research
Barton Research Building 2R28
4301 West Markham Street
Little Rock AR 72205
(501) 686-8739 PHONE
(501) 686-8987 FAX
swainfrancesl <@t> uams.edu email
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of louise renton
Sent: Thursday, February 26, 2009 3:27 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] slide storage pages
hi guys perhaps I wasn't that clear in my query. the sheets I am looking for
are for storing microscopy slides, not photo ones....sorry bout that. hope
there are still some suggestions out there
--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.
------------------------------
Message: 24
Date: Thu, 26 Feb 2009 15:18:19 +0100
From: Alexandra Meinl <alexandra.meinl <@t> gmail.com>
Subject: [Histonet] PAI-1 antibody needed
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<b7eff7a0902260618i36e6cac1hcffa71f1199a7f16 <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Hi all,
I'm looking for a PAI-1 antibody that works on FFPE mouse tissue.
Any suggestions?
Thanks in advance,
Alexandra
Alexandra MEINL
Ludwig Boltzmann Institute
for Experimental and Clinical Traumatology
Contact @ Bernhard Gottlieb University School of Dentistry,
Waehringerstr. 25a, A-1090 Vienna
------------------------------
Message: 25
Date: Thu, 26 Feb 2009 10:31:53 -0500
From: Kyla Nemitz <kynemitz <@t> travmax.com>
Subject: [Histonet] Local PRN and Contract in Northern NJ/Greater NYC
area!
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9416D9FA37C1C04FA83D69684E95D2E720BBFFEF <@t> exbk2.maxhealth.com>
Content-Type: text/plain; charset="us-ascii"
Good morning HistoNet!
One of my clients in the greater NYC Metro area is looking for a LOCAL
histo tech to work PRN (with potential for full time) starting somewhat
ASAP. If you or anyone you know is currently looking for supplemental
income in the NY/NJ area, please let me know.
ASCP is preferred, but not required.
Thank you in advance, I hope to hear from you soon!
Kyla Nemitz
TravelMax Medical Professionals - Maxim Healthcare
Tel: 888.800.1855 or 813.371.5175
Fax: 800.294.1248
Search our jobs or apply online at: www.TravelMaxAllied.com
<http://www.TravelMaxAllied.com> , www.tmaxnursing.com
------------------------------
Message: 26
Date: Thu, 26 Feb 2009 10:49:11 -0500
From: "Pam Barker" <relia1 <@t> earthlink.net>
Subject: [Histonet] RELIA Histology Careers Bulletin 2/26/09 Are you
in your dream job?
To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <E1LciU6-0006bZ-2s <@t> elasmtp-kukur.atl.sa.earthlink.net>
Content-Type: text/plain; charset="iso-8859-1"
Hi Histonetters!!
What is your dream job? I am not talking about chief chocolate taster
for Godiva
or counting your lottery winnings.
I mean are you in your dream job? If so what is it about your job that
makes it great.
If not what is it that would make your job the best?
Is it more money?
Is it more or less responsibility?
Is it a different schedule?
Is it a different location?
If you are not in your dream job let me help!!
Let?s talk about it? Maybe I can find that dream job for you.
My services are free of charge to you and completely confidential. Shoot
me an e-mail and tell me what it is that would make your dream job in
histology.
I have openings with my clients or we can customize a job search
especially for you.
All of the opportunities I represent are permanent positions with
premier companies nationwide. They offer excellent salaries benefits
and sign-on bonuses/relocation assistance.
FYI here is a list of my current openings:
HISTOLOGY MANAGEMENT
Boston, MA ? Histology Supervisor ? Research Environment
Los Angeles, CA ? Afternoon shift for National Reference Lab
Histology Supervisor Night Shift ? Ft. Lauderdale, FL
HISTOTECHS
PA- Pittsburgh, Dayshift HT or HTL required.
WA - Spokane, Clinical Position in newly remodeled lab!
CA - Los Angeles, Histotechnician/Histotechnologist ASCP cert req
CA ? San Francisco Histotechnician/Histotechnologist
MA ? North of Boston 2nd shift position
Histotechnician/Histotechnologist
MA ? Cape Cod, Histotechnician/Histotechnologist
OH - Cincinatti, Histotechnician/Histotechnologist
FL- Ft. Lauderdale 2nd and 3rd shift positions
Histotechnician/Histotechnologist
If you or any of your friends would like to chat about your dream job
and how I can help or more information about any of the positions listed
please contact me. I would be more than happy to assist you. You can
reach me at 866-607-3542 or <mailto:relia1 <@t> earthlink.net>
relia1 <@t> earthlink.net
Remember it never hurts to look?
Hope to hear from you soon . Thanks-Pam
Thank You!
Pam M. Barker
President
Relia
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
Toll Free: (866)607-3542
e-mail: <mailto:relia1 <@t> earthlink.net> relia1 <@t> earthlink.net
------------------------------
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End of Histonet Digest, Vol 63, Issue 49
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