[Histonet] Ideal fixation of mouse prostate

Vanessa J. Phelan vjp2105 <@t> columbia.edu
Thu Feb 26 14:40:46 CST 2009

Hi everyone,

I am very new to histonet, actually just joined.I hope you guys can help. I
was wondering if anyone has any experience in the fixing and processing of
mouse issues, namely prostate or bladder. I am currently trying to optomize
protocols for this as I have very recently taken on the position of
histology manager in a research lab, before this I worked with human
We have the VIP processor, and the protocol being followed at the mo is,
fixation overnight (though the tissues are tiny) and then washed in PBS and
then into 25% alcohol (sometimes over night) and then into the processor,
the cycle starts with alcohol instead of formalin.
I fear that the tissues are being overfixed and also does anyone leave their
tissues in 25% alcohol before processing?

Thanking you in advance for any input,


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