[Histonet] Ideal fixation of mouse prostate

[Vanessa J. Phelan]

Hi everyone,

I am very new to histonet, actually just joined.I hope you guys can help. I
was wondering if anyone has any experience in the fixing and processing of
mouse issues, namely prostate or bladder. I am currently trying to optomize
protocols for this as I have very recently taken on the position of
histology manager in a research lab, before this I worked with human
samples.
We have the VIP processor, and the protocol being followed at the mo is,
fixation overnight (though the tissues are tiny) and then washed in PBS and
then into 25% alcohol (sometimes over night) and then into the processor,
the cycle starts with alcohol instead of formalin.
I fear that the tissues are being overfixed and also does anyone leave their
tissues in 25% alcohol before processing?

Thanking you in advance for any input,

Nessa