[Histonet] for submission: re: quantatative fluorescent systems for tissue

[Barone, Carol]

Hitech'er's: Is there a fluorescent system (chemluninescent or bead system) for quantitatative reading of fluorescently prepared tissue sections? reader/ Scanner....and used with what kind of detection? Where do I start?

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Sent: Monday, February 23, 2009 1:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 63, Issue 43


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Today's Topics:

   1. RE: Training first time cutters (Bernice Frederick)
   2. sucrose (ejschmid <@t> ucalgary.ca)


----------------------------------------------------------------------

Message: 1
Date: Mon, 23 Feb 2009 11:08:03 -0600
From: "Bernice Frederick" <b-frederick <@t> northwestern.edu>
Subject: RE: [Histonet] Training first time cutters
To: "'Celebre Julia'" <celebrej <@t> HHSC.CA>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B604670DB9964AD18F4ABD495C2BC6F2 <@t> lurie.northwestern.edu>
Content-Type: text/plain;	charset="us-ascii"

It varies in my opinion. Some people take longer to catch on. I think
ability goes along with workload- I cut quickly and accurately whereas
others cut slower and just as accurately. I find I can vary as to tissue
also- it depends on the tech.
Bernice


Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL 
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Celebre
Julia
Sent: Monday, February 23, 2009 10:09 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Training first time cutters

>  
> 
> It's been awhile since we've had to train someone to cut from scratch and
I need your help to figure out  two things:
> 1. how long should it take a new cutter to cut 20 blocks, no levels,
nothing fancy, just one H&E per block. 
> 2. what's the average length of training  for the above to be
accomplished? how long is long enough?
> 
> Julia Celebre MLT
> Anatomic Pathology
> Hamilton General Hospital
> 905-527-0271  ext 46179
> email: celebrej <@t> hhsc.ca
> 


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------------------------------

Message: 2
Date: Mon, 23 Feb 2009 10:52:29 -0700 (MST)
From: ejschmid <@t> ucalgary.ca
Subject: [Histonet] sucrose
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <2938.136.159.74.17.1235411549.squirrel <@t> 136.159.74.17>
Content-Type: text/plain;charset=iso-8859-1

hi,

is there any reason that using ordinary table sugar is less desirable than
some formulation of Mol Bio grade sucrose when preparing a specimen for
cryrosectioning?

Eric






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End of Histonet Digest, Vol 63, Issue 43
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