[Histonet] Triple stain problem- antigen retreival conflict

[Swain, Frances L]

Dr. Chris Van der Loos has a wonderful textbook out that addresses these issues.  You can either buy the book or contact Dr. Van der Loos via the histonet.  He usually monitors this website.  He has some excellent protocols which work really nicely.

Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic Surgery
Center for Orthopaedic Research
Barton Research Building 2R28
4301 West Markham Street
Little Rock AR 72205
(501) 686-8739 PHONE
(501) 686-8987 FAX
swainfrancesl <@t> uams.edu email
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bernice Frederick
Sent: Wednesday, February 18, 2009 10:26 AM
To: 'Histonet'
Subject: [Histonet] Triple stain problem- antigen retreival conflict

Hi all,

I need some suggestion here. A researcher has requested a triple stain on
mouse tissue. We know  the antibodies will cross react but the issue is
this:

Our protocols (worked up and pathologist approved) are such that we have a
conflict of  antigen retrieval.

CD31 : proteinase K (rat ant-mouse)

MAC-3: Ph9 (rat anti- mouse)

Insulin: none ( guinea pig anti -swine)

Any help will be appreciated!!

Thanks,

Bernice

 

Bernice Frederick HTL (ASCP)

Northwestern University

Pathology Core Facility

ECOGPCO-RL 

710 N Fairbanks Court

Olson 8-421

Chicago,IL 60611

312-503-3723

 

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