[Histonet] Triple stain problem- antigen retreival conflict

[Bernice Frederick]

Hi all,

I need some suggestion here. A researcher has requested a triple stain on
mouse tissue. We know  the antibodies will cross react but the issue is
this:

Our protocols (worked up and pathologist approved) are such that we have a
conflict of  antigen retrieval.

CD31 : proteinase K (rat ant-mouse)

MAC-3: Ph9 (rat anti- mouse)

Insulin: none ( guinea pig anti -swine)

Any help will be appreciated!!

Thanks,

Bernice

 

Bernice Frederick HTL (ASCP)

Northwestern University

Pathology Core Facility

ECOGPCO-RL 

710 N Fairbanks Court

Olson 8-421

Chicago,IL 60611

312-503-3723