[Histonet] Triple stain problem- antigen retreival conflict

Bernice Frederick b-frederick <@t> northwestern.edu
Wed Feb 18 10:26:25 CST 2009

Hi all,

I need some suggestion here. A researcher has requested a triple stain on
mouse tissue. We know  the antibodies will cross react but the issue is

Our protocols (worked up and pathologist approved) are such that we have a
conflict of  antigen retrieval.

CD31 : proteinase K (rat ant-mouse)

MAC-3: Ph9 (rat anti- mouse)

Insulin: none ( guinea pig anti -swine)

Any help will be appreciated!!




Bernice Frederick HTL (ASCP)

Northwestern University

Pathology Core Facility


710 N Fairbanks Court

Olson 8-421

Chicago,IL 60611



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