[Histonet] Triple stain problem- antigen retreival conflict
b-frederick <@t> northwestern.edu
Wed Feb 18 10:26:25 CST 2009
I need some suggestion here. A researcher has requested a triple stain on
mouse tissue. We know the antibodies will cross react but the issue is
Our protocols (worked up and pathologist approved) are such that we have a
conflict of antigen retrieval.
CD31 : proteinase K (rat ant-mouse)
MAC-3: Ph9 (rat anti- mouse)
Insulin: none ( guinea pig anti -swine)
Any help will be appreciated!!
Bernice Frederick HTL (ASCP)
Pathology Core Facility
710 N Fairbanks Court
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