[Histonet] Antibody penetration problem

[Gay Rudow]

I hate to change the topic, but I have a problem with penetration of an antibody. I am using 50 μM paraffin sections and doing an antigen retrieval step of microwaving the sections in water for 5 min. I am staining by hand using the Vector ABC Elite mouse kit. The antibody is SMI 311 which I am using as a neuronal marker. Right now, my antibody penetration is only 15 μM. Does anyone have any suggestions to help me with this? Thanks!

Gay Rudow