[Histonet] query regarding IHC slide
Tony Henwood
AnthonyH <@t> chw.edu.au
Wed Feb 4 15:30:02 CST 2009
Look at the mountant you are using.
Some are not as colour-fast as one would expect.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of shazana
hilda
Sent: Wednesday, 4 February 2009 2:23 PM
To: McMahon, Loralee A; histonet forum
Subject: Re: [Histonet] query regarding IHC slide
Dear Loralee,
Unfortunately I'm using DAB and yet still faded. This is the first time
I'm facing this problem.
Thank You.
Shazana Hilda ShamsuddinUniversiti Sains Malaysia
Off. no: +609- 766 4440
Fax no: +609- 765 3370
Email: hilda_1075 <@t> yahoo.com
________________________________
From: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
To: shazana hilda <hilda_1075 <@t> yahoo.com>
Sent: Tuesday, February 3, 2009 11:47:06 PM
Subject: RE: [Histonet] query regarding IHC slide
[Histonet] query regarding IHC slide
Are you using DAB or AEC. AEC fades and DAB does not.
Loralee McMahon, HTL (ASCP)
ICC Supervisor
University of Rochester
Department of Pathology
(585) 275-7210
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of shazana
hilda
Sent: Mon 2/2/2009 9:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] query regarding IHC slide
Dear All,
Is anyone has experience with faded IHC-slide stained? I've done my IHC
stained on few markers and after 3-4 months I viewed the slide and it
seems that the stain became faded.Does anyone has opinion regarding this
matter and how to troubleshoot it?
Any response are greatly appreciated.
Regards.
Shazana Hilda ShamsuddinUniversiti Sains Malaysia,
Off. no: +609- 766 4440
Fax no: +609- 765 3370
Email: hilda_1075 <@t> yahoo.com
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