[Histonet] query regarding IHC slide

[Tony Henwood]

Look at the mountant you are using.
Some are not as colour-fast as one would expect.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of shazana
hilda
Sent: Wednesday, 4 February 2009 2:23 PM
To: McMahon, Loralee A; histonet forum
Subject: Re: [Histonet] query regarding IHC slide


Dear Loralee,
Unfortunately I'm using DAB and yet still faded. This is the first time
I'm facing this problem.

Thank You.

 
Shazana Hilda ShamsuddinUniversiti Sains Malaysia
Off. no: +609- 766 4440
Fax no: +609- 765 3370
Email: hilda_1075 <@t> yahoo.com
 




________________________________
From: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
To: shazana hilda <hilda_1075 <@t> yahoo.com>
Sent: Tuesday, February 3, 2009 11:47:06 PM
Subject: RE: [Histonet] query regarding IHC slide

[Histonet] query regarding IHC slide 
Are you using DAB or AEC.  AEC fades and DAB does not.  
 
Loralee McMahon, HTL (ASCP)
ICC Supervisor
University of Rochester 
Department of Pathology
 
(585) 275-7210
 

________________________________
 From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of shazana
hilda
Sent: Mon 2/2/2009 9:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] query regarding IHC slide


Dear All,
Is anyone has experience with faded IHC-slide stained? I've done my IHC
stained on few markers and after 3-4 months I viewed the slide and it
seems that the stain became faded.Does anyone has opinion regarding this
matter and how to troubleshoot it?

Any response are greatly appreciated.


Regards.

Shazana Hilda ShamsuddinUniversiti Sains Malaysia,
Off. no: +609- 766 4440
Fax no: +609- 765 3370
Email: hilda_1075 <@t> yahoo.com


     
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

*********************************************************************
This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
**********************************************************************