[Histonet] query regarding IHC slide

[shazana hilda]

I'm using DAB Plus chromogen from LabVision and counterstained with Harris
Hematoxylin from Sigma .Then mounted using Cytoseal XYL from
Richard-Allan Scientific.I'm dehydrated the slide through graded of alcohol then cleared in xylene prior to mounting. After done the IHC I've stored the slide in slide box at RT (about 27degrees). 

 
Shazana Hilda ShamsuddinUniversiti Sains Malaysia,
Off. no: +609- 766 4440
Fax no: +609- 765 3370
Email: hilda_1075 <@t> yahoo.com
 




________________________________
From: "anh2006 <@t> med.cornell.edu" <anh2006 <@t> med.cornell.edu>
To: shazana hilda <hilda_1075 <@t> yahoo.com>; histonet forum <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, February 4, 2009 11:37:17 AM
Subject: Re: [Histonet] query regarding IHC slide

What mounting media are you using? Are you dehydrating? How are you storing the slides? What DAB are you using (ie: source)?

-----Original Message-----
From: shazana hilda <hilda_1075 <@t> yahoo.com>

Date: Tue, 03 Feb 2009 19:23:16 
To: McMahon, Loralee A<Loralee_Mcmahon <@t> URMC.Rochester.edu>; histonet forum<histonet <@t> lists.utsouthwestern.edu>
Subject: Re: [Histonet] query regarding IHC slide


Dear Loralee,
Unfortunately I'm using DAB and yet still faded. This is the first time I'm facing this problem.

Thank You.


Shazana Hilda ShamsuddinUniversiti Sains Malaysia
Off. no: +609- 766 4440
Fax no: +609- 765 3370
Email: hilda_1075 <@t> yahoo.com





________________________________
From: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
To: shazana hilda <hilda_1075 <@t> yahoo.com>
Sent: Tuesday, February 3, 2009 11:47:06 PM
Subject: RE: [Histonet] query regarding IHC slide

[Histonet] query regarding IHC slide 
Are you using DAB or AEC.  AEC fades and DAB does not.  

Loralee McMahon, HTL (ASCP)
ICC Supervisor
University of Rochester 
Department of Pathology

(585) 275-7210


________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of shazana hilda
Sent: Mon 2/2/2009 9:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] query regarding IHC slide


Dear All,
Is anyone has experience with faded IHC-slide stained? I've done my IHC stained on few markers and after 3-4 months I viewed the slide and it seems that the stain became faded.Does anyone has opinion regarding this matter and how to troubleshoot it?

Any response are greatly appreciated.


Regards.

Shazana Hilda ShamsuddinUniversiti Sains Malaysia,
Off. no: +609- 766 4440
Fax no: +609- 765 3370
Email: hilda_1075 <@t> yahoo.com


    
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