[Histonet] RE: Bouin's rinsing protocol

Swain, Frances L SwainFrancesL <@t> uams.edu
Tue Feb 3 06:39:03 CST 2009

I have been handling Bouin fixed animal tissue for years.  We removed the Bouins picric acid yellow by submerging our specimens in 70% Alcohol saturated with Lithium Carbonate.  It usually takes overnight.  I have had PI's bring me the samples after rinsing their samples in Lithium Carbonate solution overnight, this even worked better, after rinsing with the Lithium Carbonate saturated Aqueous Solution they placed them in 70% and brought them to me for processing.  Gail 's processing is right on.  Have you seen the Animal Tissue Book that Gail and Diane Sterchi published?  It is very helpful.  I believe you can obtain it through NSH

Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic Surgery
Center for Orthopaedic Research
Barton Research Building 2R28
4301 West Markham Street
Little Rock AR 72205
(501) 686-8739 PHONE
(501) 686-8987 FAX
swainfrancesl <@t> uams.edu email

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson
Sent: Monday, February 02, 2009 3:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Bouin's rinsing protocol


I am looking for information regarding procedures for tissue handling
after Bouin's fixation, prior to processing in a standard automated
tissue processor (VIP).  We are handling pig, mouse, rat, and human
samples, and will fix in Bouin's for 48-72 hrs.  I've been told to rinse
until all yellow is washed out, and people generally rinse in 70%
alcohol or water.  Sometimes it take days to wash all of the picric acid

Thank you so much for your wealth of information!


Jennifer M. Anderson, Scientist

Halozyme Therapeutics, Inc.

11404 Sorrento Valley Road

San Diego, CA 92121


janderson <@t> halozyme.com



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