[Histonet] Bouin's rinsing protocol

[Geoff McAuliffe]

I was taught 4 changes of 50% EtOH, 1-1.5 hours each. The goal is to get 
most of the picrates out of the tissue for long-term storage (of blocks)
I don't know if one can ever get all of the yellow color out.

Geoff

Jennifer Anderson wrote:
> Hello.
>
> I am looking for information regarding procedures for tissue handling
> after Bouin's fixation, prior to processing in a standard automated
> tissue processor (VIP).  We are handling pig, mouse, rat, and human
> samples, and will fix in Bouin's for 48-72 hrs.  I've been told to rinse
> until all yellow is washed out, and people generally rinse in 70%
> alcohol or water.  Sometimes it take days to wash all of the picric acid
> out.
>
> Thank you so much for your wealth of information!
>
>  
>
> Jennifer M. Anderson, Scientist
>
> Halozyme Therapeutics, Inc.
>
> 11404 Sorrento Valley Road
>
> San Diego, CA 92121
>
> 858-704-8333
>
> janderson <@t> halozyme.com
>
>  
>
>  
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
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