[Histonet] perplexed

renafail <@t> bellsouth.net renafail <@t> bellsouth.net
Mon Feb 2 14:48:31 CST 2009

 What kind of control slides are you using?  If the marking is on the same side  as the tissue , it could prevent  your solution from getting to all your tissue.
Rena Fail

  -------------- Original message from "Angela Bitting" <akbitting <@t> geisinger.edu>: --------------

> Here's one for the Ventana BenchmarkXT users out there:
> We mount the patient tissue on the same slide with our control tissue.  The 
> control stains beautifully, but there is absolutely no staining in the patient 
> tissue. This will happen to one or two out of 30 slides on a run. It also 
> happened on two different instruments. Over the last week this has occurred 6 
> times. Different protocols, different machines.
> I tend to think its a deparaffinization issue. Can one side of a pad heat and 
> the other end not reach temp? We haven't noticed that the slides moved during 
> staining or were not seated properly on the pad.
> Help me figure this one out folks!!
> Angela Bitting, HT(ASCP)
> Technical Specialist, Histology
> Geisinger Medical Center 
> 100 N Academy Ave. MC 23-00
> Danville, PA 17822
> phone  570-214-9634
> fax  570-271-5916 
> No trees were hurt in the sending of this email
> However many electrons were severly inconvienienced!
> IMPORTANT WARNING: The information in this message (and the documents attached 
> to it, if any) is confidential and may be legally privileged. It is intended 
> solely for the addressee. Access to this message by anyone else is unauthorized. 
> If you are not the intended recipient, any disclosure, copying, distribution or 
> any action taken, or omitted to be taken, in reliance on it is prohibited and 
> may be unlawful. If you have received this message in error, please delete all 
> electronic copies of this message (and the documents attached to it, if any), 
> destroy any hard copies you may have created and notify me immediately by 
> replying to this email. Thank you.

More information about the Histonet mailing list