[Histonet] perplexed

[renafail <@t> bellsouth.net]

 What kind of control slides are you using?  If the marking is on the same side  as the tissue , it could prevent  your solution from getting to all your tissue.
Rena Fail

  -------------- Original message from "Angela Bitting" <akbitting <@t> geisinger.edu>: --------------


> Here's one for the Ventana BenchmarkXT users out there:
> 
> We mount the patient tissue on the same slide with our control tissue.  The 
> control stains beautifully, but there is absolutely no staining in the patient 
> tissue. This will happen to one or two out of 30 slides on a run. It also 
> happened on two different instruments. Over the last week this has occurred 6 
> times. Different protocols, different machines.
> I tend to think its a deparaffinization issue. Can one side of a pad heat and 
> the other end not reach temp? We haven't noticed that the slides moved during 
> staining or were not seated properly on the pad.
> Help me figure this one out folks!!
> 
> Angela Bitting, HT(ASCP)
> Technical Specialist, Histology
> Geisinger Medical Center 
> 100 N Academy Ave. MC 23-00
> Danville, PA 17822
> phone  570-214-9634
> fax  570-271-5916 
>  
> No trees were hurt in the sending of this email
> However many electrons were severly inconvienienced!
> 
> 
> 
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