[Histonet] perplexed

Bauer, Karen L. Bauer.Karen <@t> mayo.edu
Mon Feb 2 15:02:24 CST 2009

We also had this happen, and the red control box marking was on the
underside of the slide.  Seems that when the slides are packaged in the
box, the front part of the slide is pressed with the red markings on the
back of the slide in front of it.  This creates an invisible barrier on
the non-marked side and can trap the solution from spreading to the
whole slide, which prevents the patient tissue from being stained.

We got rid of our red control box slides and are just using plain
charged slides.  That problem has NEVER happened again.

There are slides with an etched box for the control tissue (etching is
on the back I believe) if you want to try those, but we decided to just
stick with what works the best.

Good Luck,


Karen L. Bauer HT(ASCP)
Histology Section Chief
Department of Pathology - Luther Hospital
Luther Midelfort - Mayo Health System
bauer.karen <@t> mayo.edu

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
renafail <@t> bellsouth.net
Sent: Monday, February 02, 2009 2:49 PM
To: Angela Bitting; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] perplexed

 What kind of control slides are you using?  If the marking is on the
same side  as the tissue , it could prevent  your solution from getting
to all your tissue.
Rena Fail

  -------------- Original message from "Angela Bitting"
<akbitting <@t> geisinger.edu>: --------------

> Here's one for the Ventana BenchmarkXT users out there:
> We mount the patient tissue on the same slide with our control tissue.

> The control stains beautifully, but there is absolutely no staining in

> the patient tissue. This will happen to one or two out of 30 slides on

> a run. It also happened on two different instruments. Over the last 
> week this has occurred 6 times. Different protocols, different
> I tend to think its a deparaffinization issue. Can one side of a pad 
> heat and the other end not reach temp? We haven't noticed that the 
> slides moved during staining or were not seated properly on the pad.
> Help me figure this one out folks!!
> Angela Bitting, HT(ASCP)
> Technical Specialist, Histology
> Geisinger Medical Center
> 100 N Academy Ave. MC 23-00
> Danville, PA 17822
> phone  570-214-9634
> fax  570-271-5916
> No trees were hurt in the sending of this email However many electrons

> were severly inconvienienced!
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