[Histonet] perplexed

Jodie Robertson jrobertson <@t> pathologysciences.com
Mon Feb 2 14:40:14 CST 2009

This is extremely familiar and we experienced a very similar situation
in our lab.  What type of slides are you currently using?  Please e-mail
me direct at jrobertson <@t> pathologysciences.com so I can be of further
assistance to you.  Thank you.

Jodie Robertson, HT(ASCP) QIHC 
Pathology Sciences Medical Group
Chico, CA  95926 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Angela
Sent: Monday, February 02, 2009 12:25 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] perplexed

Here's one for the Ventana BenchmarkXT users out there:

We mount the patient tissue on the same slide with our control tissue.
The control stains beautifully, but there is absolutely no staining in
the patient tissue. This will happen to one or two out of 30 slides on a
run. It also happened on two different instruments. Over the last week
this has occurred 6 times. Different protocols, different machines.
I tend to think its a deparaffinization issue. Can one side of a pad
heat and the other end not reach temp? We haven't noticed that the
slides moved during staining or were not seated properly on the pad.
Help me figure this one out folks!!

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
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