[Histonet] perplexed

Angela Bitting akbitting <@t> geisinger.edu
Mon Feb 2 14:25:15 CST 2009

Here's one for the Ventana BenchmarkXT users out there:

We mount the patient tissue on the same slide with our control tissue.  The control stains beautifully, but there is absolutely no staining in the patient tissue. This will happen to one or two out of 30 slides on a run. It also happened on two different instruments. Over the last week this has occurred 6 times. Different protocols, different machines.
I tend to think its a deparaffinization issue. Can one side of a pad heat and the other end not reach temp? We haven't noticed that the slides moved during staining or were not seated properly on the pad.
Help me figure this one out folks!!

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
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