[Histonet] alternates to absolute ethanol

Rene J Buesa rjbuesa <@t> yahoo.com
Fri Dec 11 11:40:15 CST 2009

The concern with methanol would be its toxicity. Methanol (TWA=200 ppm) is 5 times more toxic than ethanol (TWA=1000 ppm) and twice more toxic than 2-propanol (TWA=400ppm).
René J.

--- On Fri, 12/11/09, Owen, Michael P <Michael.Owen <@t> fda.hhs.gov> wrote:

From: Owen, Michael P <Michael.Owen <@t> fda.hhs.gov>
Subject: RE: [Histonet] alternates to absolute ethanol
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Date: Friday, December 11, 2009, 12:23 PM

Dear Michael,

Have you tried absolute methanol? The cost of the reagent should be less
expensive than absolute ethanol and is easier to obtain than absolute
ethanol (i.e. less legal restrictions than absolute ethanol that can be
diverted to non-laboratory use).

When fixing bacterial smears for a variety of staining methods, I have
discovered absolute methanol works well and is often superior to heat

Michael P. Owen, Regulatory Microbiologist
U.S. FDA Pacific Regional Lab Northwest
22201 23rd Drive SE  Bothell, WA 98021-4421
Phone: 425-483-4865     E-Mail: michael.owen <@t> fda.hhs.gov

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