[Histonet] alternates to absolute ethanol

[gayle.callis <@t> bresnan.net]

We didn't find it cheaper either. Look for a reagent alcohol mixture that is mostly ethanol. There are many mixtures of this 
Gayle Callis
Sent from my Verizon Wireless BlackBerry

-----Original Message-----
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Date: Fri, 11 Dec 2009 09:40:15 
To: Histonet<histonet <@t> lists.utsouthwestern.edu>; Michael POwen<Michael.Owen <@t> fda.hhs.gov>
Subject: RE: [Histonet] alternates to absolute ethanol

The concern with methanol would be its toxicity. Methanol (TWA=200 ppm) is 5 times more toxic than ethanol (TWA=1000 ppm) and twice more toxic than 2-propanol (TWA=400ppm).
René J.

--- On Fri, 12/11/09, Owen, Michael P <Michael.Owen <@t> fda.hhs.gov> wrote:


From: Owen, Michael P <Michael.Owen <@t> fda.hhs.gov>
Subject: RE: [Histonet] alternates to absolute ethanol
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Date: Friday, December 11, 2009, 12:23 PM


Dear Michael,

Have you tried absolute methanol? The cost of the reagent should be less
expensive than absolute ethanol and is easier to obtain than absolute
ethanol (i.e. less legal restrictions than absolute ethanol that can be
diverted to non-laboratory use).

When fixing bacterial smears for a variety of staining methods, I have
discovered absolute methanol works well and is often superior to heat
fixation.


Michael P. Owen, Regulatory Microbiologist
U.S. FDA Pacific Regional Lab Northwest
22201 23rd Drive SE  Bothell, WA 98021-4421
Phone: 425-483-4865     E-Mail: michael.owen <@t> fda.hhs.gov

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet