[Histonet] alternates to absolute ethanol

[Rene J Buesa]

Pure isopropyl = isopropanol = 2-propanol is a better dehydrant than ethanol. Try it.
René J.

--- On Fri, 12/11/09, Michael W. Folsom <mwfolsom <@t> swcp.com> wrote:


From: Michael W. Folsom <mwfolsom <@t> swcp.com>
Subject: [Histonet] alternates to absolute ethanol
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, December 11, 2009, 11:05 AM


Hi:

I want to process some plant tissue for microtomy.  When I was doing
this many years ago the protocol I used involved absolute ethanol. 
Knowing that getting ethanol that is really anhydrous is hard I was
wondering if folks were using stuff that was denatured with 55% absolute
methanol and 5% isoproponol instead.  I understand it has better shelf
life but wondered about the addition of small mounts of methanol and
isopropanol.

Also, if anybody is using this can you recommend a supplier? 


Thanks -

Mike
Rio Grand Biological

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