[Histonet] alternates to absolute ethanol
Michael W. Folsom
mwfolsom <@t> swcp.com
Fri Dec 11 10:05:04 CST 2009
Hi:
I want to process some plant tissue for microtomy. When I was doing
this many years ago the protocol I used involved absolute ethanol.
Knowing that getting ethanol that is really anhydrous is hard I was
wondering if folks were using stuff that was denatured with 55% absolute
methanol and 5% isoproponol instead. I understand it has better shelf
life but wondered about the addition of small mounts of methanol and
isopropanol.
Also, if anybody is using this can you recommend a supplier?
Thanks -
Mike
Rio Grand Biological
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