[Histonet] alternates to absolute ethanol

Michael W. Folsom mwfolsom <@t> swcp.com
Fri Dec 11 10:05:04 CST 2009


I want to process some plant tissue for microtomy.  When I was doing
this many years ago the protocol I used involved absolute ethanol. 
Knowing that getting ethanol that is really anhydrous is hard I was
wondering if folks were using stuff that was denatured with 55% absolute
methanol and 5% isoproponol instead.  I understand it has better shelf
life but wondered about the addition of small mounts of methanol and

Also, if anybody is using this can you recommend a supplier? 

Thanks -

Rio Grand Biological

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