[Histonet] Help! Losing sections from Superfrost Plus Slides

[Rene J Buesa]

Prepare a 1% aq. sol. of "Elmer's Glue" and coat the slides with it. Oven dry and use the slides as usual.
René J.

--- On Wed, 12/9/09, Angelina Fong <fong <@t> zoology.ubc.ca> wrote:


From: Angelina Fong <fong <@t> zoology.ubc.ca>
Subject: [Histonet] Help! Losing sections from Superfrost Plus Slides
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Wednesday, December 9, 2009, 2:40 PM


Hi all,

We have suddenly started losing tissue sections from our Superfrost Plus Slides. 
Our students have been cutting fixed, frozen, cryosections (20um) and thaw-mounting these onto Superfrost Plus slides.  We have suddenly started losing lots of sections from these slides (again!!).  The tissue is small - ie cross sections of frog aorta and longitudinal sections of nerves, so any lose of adhesion results in total loss of the tissue.  The odd thing is that she is not losing every section on every slide, but half to 3/4 of the sections are falling off within the first rinse.  The sections are from the same tissue block on the same slide while some falls and others don't.

We are at a lose as to what we can do to rescue these sections.

Does anyone know if there is any way to coat the slides in some solution with the tissue on them to help improve the adhesion without losing the ability to do immunofluorescence?

Any further advice on cutting / drying protocols are welcomed. 
This keeps happening and the inconsistency of it has us so frustrated with this that we are thinking of going back to subbing our own slides.

Thanks for your help!

Angelina


-- ~~o~~o~~o~~o~~o~~o~~o~~o~~o~~o

Angelina Y. Fong, Ph.D.
Department of Zoology
Biological Sciences Building
6270 University Boulevard
University of British Columbia
Vancouver, BC, V6T 1Z4
Canada 
Ph:  (604) 822-5799
Fax: (604) 822-2416
Email: fong <@t> zoology.ubc.ca



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