[Histonet] Help! Losing sections from Superfrost Plus Slides

[CHRISTIE GOWAN]

I would suggest you check the lot#. You may have received a bad lot and need to get them replaced by the vendor. 
 
>> 
> Hi all,
> 
> We have suddenly started losing tissue sections from our Superfrost Plus Slides. 
> Our students have been cutting fixed, frozen, cryosections (20um) and thaw-mounting these onto Superfrost Plus slides.  We have suddenly started losing lots of sections from these slides (again!!).  The tissue is small - ie cross sections of frog aorta and longitudinal sections of nerves, so any lose of adhesion results in total loss of the tissue.  The odd thing is that she is not losing every section on every slide, but half to 3/4 of the sections are falling off within the first rinse.  The sections are from the same tissue block on the same slide while some falls and others don't.
> 
> We are at a lose as to what we can do to rescue these sections.
> 
> Does anyone know if there is any way to coat the slides in some solution with the tissue on them to help improve the adhesion without losing the ability to do immunofluorescence?
> 
> Any further advice on cutting / drying protocols are welcomed. 
> This keeps happening and the inconsistency of it has us so frustrated with this that we are thinking of going back to subbing our own slides.
> 
> Thanks for your help!
> 
> Angelina
> 
> 
> -- ~~o~~o~~o~~o~~o~~o~~o~~o~~o~~o
> 
> Angelina Y. Fong, Ph.D.
> Department of Zoology
> Biological Sciences Building
> 6270 University Boulevard
> University of British Columbia
> Vancouver, BC, V6T 1Z4
> Canada 
> Ph:  (604) 822-5799
> Fax: (604) 822-2416
> Email: fong <@t> zoology.ubc.ca
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet