[Histonet] Help! Losing sections from Superfrost Plus Slides
fong <@t> zoology.ubc.ca
Wed Dec 9 13:40:50 CST 2009
We have suddenly started losing tissue sections from our Superfrost Plus
Our students have been cutting fixed, frozen, cryosections (20um) and
thaw-mounting these onto Superfrost Plus slides. We have suddenly
started losing lots of sections from these slides (again!!). The tissue
is small - ie cross sections of frog aorta and longitudinal sections of
nerves, so any lose of adhesion results in total loss of the tissue.
The odd thing is that she is not losing every section on every slide,
but half to 3/4 of the sections are falling off within the first rinse.
The sections are from the same tissue block on the same slide while some
falls and others don't.
We are at a lose as to what we can do to rescue these sections.
Does anyone know if there is any way to coat the slides in some solution
with the tissue on them to help improve the adhesion without losing the
ability to do immunofluorescence?
Any further advice on cutting / drying protocols are welcomed.
This keeps happening and the inconsistency of it has us so frustrated
with this that we are thinking of going back to subbing our own slides.
Thanks for your help!
Angelina Y. Fong, Ph.D.
Department of Zoology
Biological Sciences Building
6270 University Boulevard
University of British Columbia
Vancouver, BC, V6T 1Z4
Ph: (604) 822-5799
Fax: (604) 822-2416
Email: fong <@t> zoology.ubc.ca
More information about the Histonet