[Histonet] Help! Losing sections from Superfrost Plus Slides

[Angelina Fong]

Hi all,

We have suddenly started losing tissue sections from our Superfrost Plus 
Slides. 

Our students have been cutting fixed, frozen, cryosections (20um) and 
thaw-mounting these onto Superfrost Plus slides.  We have suddenly 
started losing lots of sections from these slides (again!!).  The tissue 
is small - ie cross sections of frog aorta and longitudinal sections of 
nerves, so any lose of adhesion results in total loss of the tissue.  
The odd thing is that she is not losing every section on every slide, 
but half to 3/4 of the sections are falling off within the first rinse.  
The sections are from the same tissue block on the same slide while some 
falls and others don't.

We are at a lose as to what we can do to rescue these sections.

Does anyone know if there is any way to coat the slides in some solution 
with the tissue on them to help improve the adhesion without losing the 
ability to do immunofluorescence?

Any further advice on cutting / drying protocols are welcomed. 

This keeps happening and the inconsistency of it has us so frustrated 
with this that we are thinking of going back to subbing our own slides.

Thanks for your help!

Angelina


-- 
~~o~~o~~o~~o~~o~~o~~o~~o~~o~~o

Angelina Y. Fong, Ph.D.
Department of Zoology
Biological Sciences Building
6270 University Boulevard
University of British Columbia
Vancouver, BC, V6T 1Z4
Canada 

Ph:  (604) 822-5799
Fax: (604) 822-2416
Email: fong <@t> zoology.ubc.ca