[Histonet] opinion please

[Akemi Allison]

Sorry I made a type "O" I meant to say Also, manufacturer's will  
revalidate antibodies when they are close to their expiration date  
and if that antibody has the same sensitivity.  They will extend the  
shelf-life date.
>


Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: akemiat3377 <@t> yahoo.com

On Dec 7, 2009, at 5:02 PM, Akemi Allison wrote:

>
> I agree with Tim.  This is a very common practice for antibody  
> manufacturer's.   Also, manufacturer's will revalidate antibodies  
> when they are close to their exploration date and if that antibody  
> has the same sensitivity.  They will extend the shelf-life date.
>
> Akemi Allison BS, HT (ASCP) HTL
> Director
> Phoenix Lab Consulting
> Tele: 408.335.9994
> E-Mail: akemiat3377 <@t> yahoo.com
>
> On Dec 7, 2009, at 2:32 PM, Morken, Tim wrote:
>
>> Kim,
>>
>> Test a random sampling (10%) of the antibodies and see if they  
>> work. If so, then they are most likely fine. Document the test.  
>> Antibodies are very robust and the warmup probably won't damage  
>> many, if any, of them.
>>
>> BTW, When I worked for an antibody manufacturer I did a test once  
>> as part of a stability problem investigation and left six  
>> different antibodies in a 37C oven for up to 4 months. We tested a  
>> sample of each weekly. All worked fine and some even worked better  
>> even after 4 months!
>>
>> The issue I would be concerned about is bacterial growth in any of  
>> them. Keep an eye on that.
>>
>> Tim Morken
>> Supervisor, Histology / IPOX
>> UCSF Medical Center
>> San Francisco, CA
>>
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet- 
>> bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Merriam
>> Sent: Monday, December 07, 2009 6:51 AM
>> To: Histonet
>> Subject: [Histonet] opinion please
>>
>> Help!
>>
>> My refrigerator died over the weekend.  This fridge had most of my  
>> antibodies and IHC reagents.  When I came in, the temperature  
>> inside the fridge was a balmy 37C!
>>
>> Do you think any of my reagents are still usable?
>>
>> Kim
>>  Kim Merriam, MA, HT(ASCP)QIHC
>> Cambridge, MA
>>
>>
>>
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>