[Histonet] opinion please

[Akemi Allison]

I agree with Tim.  This is a very common practice for antibody  
manufacturer's.   Also, manufacturer's will revalidate antibodies  
when they are close to their exploration date and if that antibody  
has the same sensitivity.  They will extend the shelf-life date.

Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: akemiat3377 <@t> yahoo.com

On Dec 7, 2009, at 2:32 PM, Morken, Tim wrote:

> Kim,
>
> Test a random sampling (10%) of the antibodies and see if they  
> work. If so, then they are most likely fine. Document the test.  
> Antibodies are very robust and the warmup probably won't damage  
> many, if any, of them.
>
> BTW, When I worked for an antibody manufacturer I did a test once  
> as part of a stability problem investigation and left six different  
> antibodies in a 37C oven for up to 4 months. We tested a sample of  
> each weekly. All worked fine and some even worked better even after  
> 4 months!
>
> The issue I would be concerned about is bacterial growth in any of  
> them. Keep an eye on that.
>
> Tim Morken
> Supervisor, Histology / IPOX
> UCSF Medical Center
> San Francisco, CA
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet- 
> bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Merriam
> Sent: Monday, December 07, 2009 6:51 AM
> To: Histonet
> Subject: [Histonet] opinion please
>
> Help!
>
> My refrigerator died over the weekend.  This fridge had most of my  
> antibodies and IHC reagents.  When I came in, the temperature  
> inside the fridge was a balmy 37C!
>
> Do you think any of my reagents are still usable?
>
> Kim
>  Kim Merriam, MA, HT(ASCP)QIHC
> Cambridge, MA
>
>
>
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