[Histonet] opinion please

[Bader Siddiki]

Hello
I have the same experience.
When I worked for a company and right after 9/11, we shipped antibodies to
Israel via Post office at ambient temp.
This package came back after 6-8 weeks or may be longer.
We do not know how they were stored at post office.
We tested the antibodies and could not find differences in the activity
using IHC.
Bader

On Mon, Dec 7, 2009 at 4:02 PM, Akemi Allison <akemiat3377 <@t> yahoo.com> wrote:

>
> I agree with Tim.  This is a very common practice for antibody
> manufacturer's.   Also, manufacturer's will revalidate antibodies when they
> are close to their exploration date and if that antibody has the same
> sensitivity.  They will extend the shelf-life date.
>
> Akemi Allison BS, HT (ASCP) HTL
> Director
> Phoenix Lab Consulting
> Tele: 408.335.9994
> E-Mail: akemiat3377 <@t> yahoo.com
>
> On Dec 7, 2009, at 2:32 PM, Morken, Tim wrote:
>
>  Kim,
>>
>> Test a random sampling (10%) of the antibodies and see if they work. If
>> so, then they are most likely fine. Document the test. Antibodies are very
>> robust and the warmup probably won't damage many, if any, of them.
>>
>> BTW, When I worked for an antibody manufacturer I did a test once as part
>> of a stability problem investigation and left six different antibodies in a
>> 37C oven for up to 4 months. We tested a sample of each weekly. All worked
>> fine and some even worked better even after 4 months!
>>
>> The issue I would be concerned about is bacterial growth in any of them.
>> Keep an eye on that.
>>
>> Tim Morken
>> Supervisor, Histology / IPOX
>> UCSF Medical Center
>> San Francisco, CA
>>
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-
>> bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Merriam
>> Sent: Monday, December 07, 2009 6:51 AM
>> To: Histonet
>> Subject: [Histonet] opinion please
>>
>> Help!
>>
>> My refrigerator died over the weekend.  This fridge had most of my
>> antibodies and IHC reagents.  When I came in, the temperature inside the
>> fridge was a balmy 37C!
>>
>> Do you think any of my reagents are still usable?
>>
>> Kim
>>  Kim Merriam, MA, HT(ASCP)QIHC
>> Cambridge, MA
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
If any Q's please feel free to contact us
Have a nice day/weekend
Mit freundlichen Grüßen / With Kind Regards /
avec l'aimable ce qui concerne
Met vriendelijke groeten
種とについて
Bader
Bader B Siddiki, PhD
Executive director,
Research and development
ImmunoBioScience corp.
Phone: + 425 367 4601
Phone: + 425 514 3761
Fax: + 425 367 4817
cell (mobile) phone: + 425 314 0199
e-mail address: baderbo <@t> gmail.com
Web site: www.immunobioscience.com
Marketing: phone: + 650 343 IBSC (4272)
               E-mail: anitaIBSC <@t> aol.com