[Histonet] opinion please

Morken, Tim Timothy.Morken <@t> ucsfmedctr.org
Mon Dec 7 15:32:42 CST 2009


Test a random sampling (10%) of the antibodies and see if they work. If so, then they are most likely fine. Document the test. Antibodies are very robust and the warmup probably won't damage many, if any, of them. 

BTW, When I worked for an antibody manufacturer I did a test once as part of a stability problem investigation and left six different antibodies in a 37C oven for up to 4 months. We tested a sample of each weekly. All worked fine and some even worked better even after 4 months!

The issue I would be concerned about is bacterial growth in any of them. Keep an eye on that.

Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA  

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Monday, December 07, 2009 6:51 AM
To: Histonet
Subject: [Histonet] opinion please


My refrigerator died over the weekend.  This fridge had most of my antibodies and IHC reagents.  When I came in, the temperature inside the fridge was a balmy 37C!

Do you think any of my reagents are still usable?

 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 

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