[Histonet] slide handling for special stains

Rene J Buesa rjbuesa <@t> yahoo.com
Mon Dec 7 08:24:40 CST 2009

No, it is not a good idea. In many occasions leaving the dewaxed sections in DW for prolonged periods of time can cause the section to peel off (not always, but sometimes).
The idea is to dewax and do the special stains immediately after.
René J.

--- On Mon, 12/7/09, Charles, Roger <rcharles <@t> state.pa.us> wrote:

From: Charles, Roger <rcharles <@t> state.pa.us>
Subject: [Histonet] slide handling for special stains
To: "Histonet (histonet <@t> lists.utsouthwestern.edu)" <histonet <@t> lists.utsouthwestern.edu>
Date: Monday, December 7, 2009, 8:19 AM

Hello all,
I need some information on special stains in regards to handling of slides.   Would it be good practice to deparaffinize and rehydrate slides then allow them to sit in water over night or over the weekend before doing the actual staining?

Roger Charles
Pennsylvania Veterinary Laboratory
2305 N Cameron St
Harrisburg, PA 17110
rcharles <@t> state.pa.us<mailto:rcharles <@t> state.pa.us>

No trees were hurt in the sending of this email, However many electrons were severely inconvenienced!

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu


More information about the Histonet mailing list