[Histonet] slide handling for special stains

[Lynette Pavelich]

My teacher, long retired Miss Mavis Marie, a MT that loved Histology, taught us that if we needed to do the staining the next day, to leave them in 70% alcohol.  We did find that sometimes, not always, if left in distilled H2O overnight, it would wash off, but never did when left in 70%.

Hope this helped,
Lynette

>>> Rene J Buesa <rjbuesa <@t> yahoo.com> 12/7/2009 9:24 AM >>>
No, it is not a good idea. In many occasions leaving the dewaxed sections in DW for prolonged periods of time can cause the section to peel off (not always, but sometimes).
The idea is to dewax and do the special stains immediately after.
René J.

--- On Mon, 12/7/09, Charles, Roger <rcharles <@t> state.pa.us> wrote:


From: Charles, Roger <rcharles <@t> state.pa.us>
Subject: [Histonet] slide handling for special stains
To: "Histonet (histonet <@t> lists.utsouthwestern.edu)" <histonet <@t> lists.utsouthwestern.edu>
Date: Monday, December 7, 2009, 8:19 AM


Hello all,
I need some information on special stains in regards to handling of slides.   Would it be good practice to deparaffinize and rehydrate slides then allow them to sit in water over night or over the weekend before doing the actual staining?
Thanks
Roger

Roger Charles
Microbiologist
Pennsylvania Veterinary Laboratory
2305 N Cameron St
Harrisburg, PA 17110
717-787-8808
rcharles <@t> state.pa.us<mailto:rcharles <@t> state.pa.us>


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