[Histonet] slide handling for special stains

Kim.Donadio <@t> bhcpns.org Kim.Donadio <@t> bhcpns.org
Mon Dec 7 08:15:54 CST 2009

I wouldn't make that a habit. It eventually saturates the cells and then 
they fall off like the previous poster mentioned. Also, especially leaving 
them in water for a whole weekend you risk contamination. Not good 
practice. If you absolutely have to leave them in something after they 
have been deparaffinized I would suggest 95% alcohol. With that said, I 
think best practice would be to leave them paraffinized until you have 
time to do the stains. 

Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996

"Charles, Roger" <rcharles <@t> state.pa.us> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
12/07/2009 07:19 AM

"Histonet (histonet <@t> lists.utsouthwestern.edu)" 
<histonet <@t> lists.utsouthwestern.edu>

[Histonet] slide handling for special stains

Hello all,
I need some information on special stains in regards to handling of 
slides.   Would it be good practice to deparaffinize and rehydrate slides 
then allow them to sit in water over night or over the weekend before 
doing the actual staining?

Roger Charles
Pennsylvania Veterinary Laboratory
2305 N Cameron St
Harrisburg, PA 17110
rcharles <@t> state.pa.us<mailto:rcharles <@t> state.pa.us>

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