[Histonet] IF colocalization with 2 rabbit antibodies
Samuel_Perry <@t> DFCI.HARVARD.EDU
Thu Dec 3 11:30:47 CST 2009
I know this might be a dumb question.
Has anyone been able to perform CO-IF staining with two rabbit antibodies with
confidence in specificity of the signals? I am worried that using the same
specie antibody with cause specificity issues. I'm interested in staining
p-S6RP and p-44/42 (two rabbit mAb from cell signaling) on the same section of
Any suggestions would be very helpful even if people have tried and failed; that
would be good to know.
Research Tech DFCI
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