[Histonet] regarding using neg controls for H.pylori

[Horn, Hazel V]

We do this as well.   Each block has a pt. positive slide and a pt.
negative slide for that block.  If multiple antibodies are ordered on
the same block, we usually will have one negative mouse and one negative
rabbit for the block. 

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's Way    Slot 820
Little Rock, AR   72202
 
phone   501.364.4240
fax        501.364.3155
 
visit us on the web at:    www.archildrens.org
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Kim.Donadio <@t> bhcpns.org
Sent: Thursday, December 03, 2009 11:03 AM
To: srishan <@t> mail.holyname.org
Cc: histonet <@t> lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] regarding using neg controls for H.pylori

Here we run a pos control which is on each patient slide and one Neg 
control for every block such as "A, B, Etc"  I believe this is the CAP 
recommendation

You can look at all the specific guidelines by going to the CAP website 
and looking at questions ANP.22550 and ANP.22570

Hope this helps


Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996



srishan <@t> mail.holyname.org 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
12/03/2009 10:48 AM

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Subject
[Histonet] regarding using neg controls for H.pylori






Good Morning,

I am sure this question has popped up before, but,  when you are running

H.pylori by immunohistochemistry do you have to run a patient pos and 
patient neg for each patient.  If you have 10 patients do the run
consist 
of 20 slides each patient having a pos and neg. What does the CAP
suggest 
us to do.?  We have a debate going regarding this issue and I would like

clarification.  I understand when we do 2 slides each  there is only one

billable item and the neg is using reagents but cannot be reimbursed.

Thanks

Mala

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