[Histonet] procedure for osmium and potassium chromate?

[Geoff McAuliffe]

OK, light microscopy.
I think frozen sections of formalin fixed (formalin with calcium) 
material is the way to go here. Wash out the formalin as it will react 
with the dicrhomate then "fix" with Os or Os-dichromate.
Altman's fix is equal parts of 2% aqueous osmium and 5% aqueous 
potassium dichromate. There are many variations on this mix, I doubt if 
concentration matters much. Fix sections on slides for an hour or two, 
wash well.
I have fixed mouse kidney with osmium-dichromate for paraffin embedding. 
The tissue is VERY brittle and hard to cut. I would not expect good results.

Geoff

Liz Chlipala wrote:
> Light Micro.  I have done this before and it turned out great on some
> mouse muscle, but I misplaced my procedure.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Manager
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, Colorado 80308
> office (303) 682-3949 
> fax (303) 682-9060
> www.premierlab.com
>  
>  
> Ship to Address:
> 1567 Skyway Drive, Unit E
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>
> -----Original Message-----
> From: Geoff McAuliffe [mailto:mcauliff <@t> umdnj.edu] 
> Sent: Wednesday, December 02, 2009 1:41 PM
> To: Liz Chlipala
> Cc: histonet <@t> pathology.swmed.edu
> Subject: Re: [Histonet] procedure for osmium and potassium chromate?
>
> Dalton's Chrome-osmium fix was popular for awhile in the 1950's. You 
> will need to look in an older methods book. The combo of dichromate and 
> osmium will not make your safety officer happy. There are many ways to 
> stain lipids with osmium, alone or mixed with something else. Is this 
> for EM or LM?
>
> Geoff
>
> Liz Chlipala wrote:
>   
>> I'm trying to find a procedure for staining fat in tissues with osmium
>> and I think potassium dichromate, does anyone out there have this
>> protocol available.  I have found it before on the web but for some
>> reason I can't  find it this time.
>>
>>  
>>
>> Liz
>>
>>  
>>
>> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
>>
>> Manager
>>
>> Premier Laboratory, LLC
>>
>> PO Box 18592
>>
>> Boulder, Colorado 80308
>>
>> office (303) 682-3949 
>>
>> fax (303) 682-9060
>>
>> www.premierlab.com
>>
>>  
>>
>>  
>>
>> Ship to Address:
>>
>> 1567 Skyway Drive, Unit E
>>
>> Longmont, Colorado 80504
>>
>>  
>>
>> _______________________________________________
>> Histonet mailing list
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>>
>>
>>   
>>     
>
>
>   


-- 
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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