[Histonet] procedure for osmium and potassium chromate?

[Liz Chlipala]

Light Micro.  I have done this before and it turned out great on some
mouse muscle, but I misplaced my procedure.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: Geoff McAuliffe [mailto:mcauliff <@t> umdnj.edu] 
Sent: Wednesday, December 02, 2009 1:41 PM
To: Liz Chlipala
Cc: histonet <@t> pathology.swmed.edu
Subject: Re: [Histonet] procedure for osmium and potassium chromate?

Dalton's Chrome-osmium fix was popular for awhile in the 1950's. You 
will need to look in an older methods book. The combo of dichromate and 
osmium will not make your safety officer happy. There are many ways to 
stain lipids with osmium, alone or mixed with something else. Is this 
for EM or LM?

Geoff

Liz Chlipala wrote:
> I'm trying to find a procedure for staining fat in tissues with osmium
> and I think potassium dichromate, does anyone out there have this
> protocol available.  I have found it before on the web but for some
> reason I can't  find it this time.
>
>  
>
> Liz
>
>  
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
>
> Manager
>
> Premier Laboratory, LLC
>
> PO Box 18592
>
> Boulder, Colorado 80308
>
> office (303) 682-3949 
>
> fax (303) 682-9060
>
> www.premierlab.com
>
>  
>
>  
>
> Ship to Address:
>
> 1567 Skyway Drive, Unit E
>
> Longmont, Colorado 80504
>
>  
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>   


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
**********************************************