[Histonet] procedure for osmium and potassium chromate?
cpyse <@t> x-celllab.com
Wed Dec 2 15:05:59 CST 2009
OSMIUM TETROXIDE FOR FAT (Paraffin SECTIONS)
2.5% Potassium Dichromate Solution Stock
1% Osmium Tetroxide Solution Stock
Potassium Dichromate-Osmium Tetroxide Solution Working
Potassium Dichromate Stock 50 ml
Osmium Tetroxide stock 50 ml
1. Place formalin fixed specimen, no thicker than 4mm, in working potassium
dichromate-osmium tetroxide solution for up to 16 hours.
2. Wash specimen in running water for 2 hours.
3. Process tissue, embed in paraffin and cut at 4 microns.
4. Stain in routine H&E staining setup, than mount.
Fat - black
Background - same as H&E stain.
Hope this helps. If you have any questions please let me know.
Cindy Pyse, CLT, HT (ASCP)
e-mail cpyse <@t> x-celllab.com
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Wednesday, December 02, 2009 3:13 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] procedure for osmium and potassium chromate?
I'm trying to find a procedure for staining fat in tissues with osmium
and I think potassium dichromate, does anyone out there have this
protocol available. I have found it before on the web but for some
reason I can't find it this time.
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504
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