[Histonet] Trophic factors staining on brain sections

[TF]

Hi all
to stain out the trophic factors on brain sections, such as BDNFm, IGF1, NGF, CNTF, GDNF

will these molecules "leak" out during staining? so I should not use triton and try to shorten the time of staining?
Or I need a long time incubation to stain out the "low" concentration small molecules?

thanks!


2009-12-02 



TF 
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