Hi all to stain out the trophic factors on brain sections, such as BDNFm, IGF1, NGF, CNTF, GDNF will these molecules "leak" out during staining? so I should not use triton and try to shorten the time of staining? Or I need a long time incubation to stain out the "low" concentration small molecules? thanks! 2009-12-02 TF