[Histonet] procedure for osmium and potassium chromate?

Rene J Buesa rjbuesa <@t> yahoo.com
Wed Dec 2 15:15:03 CST 2009

I really would suggest you to find an alternative procedure.
Osmium tetroxyde is EXTREMELY dangerous and has to be used under extreme precautions.
I don't think the results are worth the effort given the great dangers it poses.
I for one would not share the procedure with you.
René J.

--- On Wed, 12/2/09, Liz Chlipala <liz <@t> premierlab.com> wrote:

From: Liz Chlipala <liz <@t> premierlab.com>
Subject: [Histonet] procedure for osmium and potassium chromate?
To: histonet <@t> pathology.swmed.edu
Date: Wednesday, December 2, 2009, 3:13 PM

I'm trying to find a procedure for staining fat in tissues with osmium
and I think potassium dichromate, does anyone out there have this
protocol available.  I have found it before on the web but for some
reason I can't  find it this time.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC


Premier Laboratory, LLC

PO Box 18592

Boulder, Colorado 80308

office (303) 682-3949 

fax (303) 682-9060


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