[Histonet] procedure for osmium and potassium chromate?
Geoff McAuliffe
mcauliff <@t> umdnj.edu
Wed Dec 2 14:40:54 CST 2009
Dalton's Chrome-osmium fix was popular for awhile in the 1950's. You
will need to look in an older methods book. The combo of dichromate and
osmium will not make your safety officer happy. There are many ways to
stain lipids with osmium, alone or mixed with something else. Is this
for EM or LM?
Geoff
Liz Chlipala wrote:
> I'm trying to find a procedure for staining fat in tissues with osmium
> and I think potassium dichromate, does anyone out there have this
> protocol available. I have found it before on the web but for some
> reason I can't find it this time.
>
>
>
> Liz
>
>
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
>
> Manager
>
> Premier Laboratory, LLC
>
> PO Box 18592
>
> Boulder, Colorado 80308
>
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>
> fax (303) 682-9060
>
> www.premierlab.com
>
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--
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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voice: (732)-235-4583
mcauliff <@t> umdnj.edu
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