[Histonet] water on slides?

[Va Paula Sicurello]

I'm getting the same eosin bleeding and water on the slides.  I'm using Hemo-D and CitriSolv.  I have changed the reagents and am still getting the eosin bleeding, the water on the slide is a new phenomenon.

Any suggestions?


Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF) 
Core for Micro Imaging(C-MI)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397

C-MI for your imaging needs.


--- On Mon, 8/24/09, Angela Bitting <akbitting <@t> geisinger.edu> wrote:

> From: Angela Bitting <akbitting <@t> geisinger.edu>
> Subject: Re: [Histonet] water on slides?
> To: histonet <@t> lists.utsouthwestern.edu, "Tina J. Hayes" <Tina.Hayes <@t> va.gov>
> Date: Monday, August 24, 2009, 6:00 PM
> We are having a very similar problem
> too. We change our alcohols and Histoclear like mad, but are
> having eosin bleeding out of the sections. I'm starting to
> wonder if it is the mounting media on our glass
> coverslipper. I ordered new bottle gaskets, so I guess I'll
> see what happens then.
> 
> Angela Bitting, HT(ASCP)
> Technical Specialist, Histology
> Geisinger Medical Center 
> 100 N Academy Ave. MC 23-00
> Danville, PA 17822
> phone  570-214-9634
> fax  570-271-5916 
>  
> No trees were hurt in the sending of this email
> However many electrons were severly inconvienienced!
> 
> 
> >>> "Hayes, Tina J." <Tina.Hayes <@t> va.gov>
> 8/24/2009 12:41 PM >>>
> We are having a problem with what seems to be water on our
> slides.  We
> have had very high humidity levels in the air.  It
> seems that we look at
> the slides before taking them to the pathology office, one
> pathologist
> reviews them and sees little or no water droplets, but as
> they sit and
> another pathologist reads them later, like the following
> day, the
> droplets are very apparent.
> 
>  
> 
> We use Clearite-3.  We have no xylene in our
> lab.  We also coverslip
> with Permount.
> 
>  
> 
> Is it possible that the clearite-3 and/or Permount is
> absorbing moisture
> from the atmosphere to the slides?
> 
> And if so, do you have any suggestions for combating this
> issue?
> 
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