[Histonet] water on slides?

[Angela Bitting]

We are having a very similar problem too. We change our alcohols and Histoclear like mad, but are having eosin bleeding out of the sections. I'm starting to wonder if it is the mounting media on our glass coverslipper. I ordered new bottle gaskets, so I guess I'll see what happens then.

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
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>>> "Hayes, Tina J." <Tina.Hayes <@t> va.gov> 8/24/2009 12:41 PM >>>
We are having a problem with what seems to be water on our slides.  We
have had very high humidity levels in the air.  It seems that we look at
the slides before taking them to the pathology office, one pathologist
reviews them and sees little or no water droplets, but as they sit and
another pathologist reads them later, like the following day, the
droplets are very apparent.

 

We use Clearite-3.  We have no xylene in our lab.  We also coverslip
with Permount.

 

Is it possible that the clearite-3 and/or Permount is absorbing moisture
from the atmosphere to the slides?

And if so, do you have any suggestions for combating this issue?

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