[Histonet] water on slides?

Anthony Reilly Tony_Reilly <@t> health.qld.gov.au
Mon Aug 24 18:14:16 CDT 2009

If your solutions are clean the cause may be that the level of the water wash station on your stainer is higher than the level of the subsequent alcohol container which means that some water will not be removed from the top of the slide.  This is common with running water washes on stainers as the water pressure will elevate the level.  A common artifact appears as a blue stripe down the slide caused by the water running down and removing the eosin.
Tony Reilly

Chief Scientist
Anatomical Pathology
Pathology Queensland
Level 1, Building 15
Princess Alexandra Hospital
Ipswich Rd, 
Woolloongabba Q 4102
Ph: 07 32402412
Fax:07 32402930
tony_reilly <@t> health.qld.gov.au

>>> Va Paula Sicurello <vapatpxs <@t> yahoo.com> 25/08/2009 7:30 am >>>
I'm getting the same eosin bleeding and water on the slides.  I'm using Hemo-D and CitriSolv.  I have changed the reagents and am still getting the eosin bleeding, the water on the slide is a new phenomenon.

Any suggestions?

Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF) 
Core for Micro Imaging(C-MI)
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397

C-MI for your imaging needs.

--- On Mon, 8/24/09, Angela Bitting <akbitting <@t> geisinger.edu> wrote:

> From: Angela Bitting <akbitting <@t> geisinger.edu>
> Subject: Re: [Histonet] water on slides?
> To: histonet <@t> lists.utsouthwestern.edu, "Tina J. Hayes" <Tina.Hayes <@t> va.gov>
> Date: Monday, August 24, 2009, 6:00 PM
> We are having a very similar problem
> too. We change our alcohols and Histoclear like mad, but are
> having eosin bleeding out of the sections. I'm starting to
> wonder if it is the mounting media on our glass
> coverslipper. I ordered new bottle gaskets, so I guess I'll
> see what happens then.
> Angela Bitting, HT(ASCP)
> Technical Specialist, Histology
> Geisinger Medical Center 
> 100 N Academy Ave. MC 23-00
> Danville, PA 17822
> phone  570-214-9634
> fax  570-271-5916 
> No trees were hurt in the sending of this email
> However many electrons were severly inconvienienced!
> >>> "Hayes, Tina J." <Tina.Hayes <@t> va.gov>
> 8/24/2009 12:41 PM >>>
> We are having a problem with what seems to be water on our
> slides.  We
> have had very high humidity levels in the air.  It
> seems that we look at
> the slides before taking them to the pathology office, one
> pathologist
> reviews them and sees little or no water droplets, but as
> they sit and
> another pathologist reads them later, like the following
> day, the
> droplets are very apparent.
> We use Clearite-3.  We have no xylene in our
> lab.  We also coverslip
> with Permount.
> Is it possible that the clearite-3 and/or Permount is
> absorbing moisture
> from the atmosphere to the slides?
> And if so, do you have any suggestions for combating this
> issue?
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