[Histonet] destaining cyto slides for immuno

[Karin Groeger]

We do the same at USLabs.

Karin Groeger

Histology Supervisor

US LABS, Irvine,CA

949-450-0145 ext. 649


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Friday, August 14, 2009 7:28 AM
To: 'Jennifer Campbell'
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: AW: [Histonet] destaining cyto slides for immuno

We do p16-stain on PAP-smears on the Ventana Benchmark. The retrieval step
removes the dye, so there's no extra procedure needed.

PAP smears are not formalin fixed specimen, but a mild antigen retrieval
improves the result and destains the slides.

Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Jennifer
Campbell
Gesendet: Freitag, 14. August 2009 16:13
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] destaining cyto slides for immuno

Hi All,
 
  How would you go about destaining a cyto slide and running a
CKAE1/AE3?  I was told that acid alcohol to remove the stain beforehand
may not be necessary as heat retrieval may remove it adequately enough.
Does anyone have any recommendations?
 
Thanks,
 
Jen
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