[Histonet] destaining cyto slides for immuno

Rene J Buesa rjbuesa <@t> yahoo.com
Fri Aug 14 10:00:54 CDT 2009

Heat retrieval will not destain the smear. Use acid alcohol followed by alcohol and water and then do the IHC procedure.
You will NOT need HIER unless your smears were fixed with formalin. Since they usually are fixed only with alcohol, you can go directly to the IHC procedure.
René J.

--- On Fri, 8/14/09, Jennifer Campbell <jcampbell <@t> vdxpathology.com> wrote:

From: Jennifer Campbell <jcampbell <@t> vdxpathology.com>
Subject: [Histonet] destaining cyto slides for immuno
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, August 14, 2009, 10:12 AM

Hi All,

  How would you go about destaining a cyto slide and running a
CKAE1/AE3?  I was told that acid alcohol to remove the stain beforehand
may not be necessary as heat retrieval may remove it adequately enough.
Does anyone have any recommendations?


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