AW: [Histonet] destaining cyto slides for immuno

Gudrun Lang gu.lang <@t>
Fri Aug 14 09:27:54 CDT 2009

We do p16-stain on PAP-smears on the Ventana Benchmark. The retrieval step
removes the dye, so there's no extra procedure needed.

PAP smears are not formalin fixed specimen, but a mild antigen retrieval
improves the result and destains the slides.


-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] Im Auftrag von Jennifer
Gesendet: Freitag, 14. August 2009 16:13
An: histonet <@t>
Betreff: [Histonet] destaining cyto slides for immuno

Hi All,
  How would you go about destaining a cyto slide and running a
CKAE1/AE3?  I was told that acid alcohol to remove the stain beforehand
may not be necessary as heat retrieval may remove it adequately enough.
Does anyone have any recommendations?
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